Catalog Number - 60022-05P-2

60022-05P-2 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for challenging separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.

With a 5 µm particle size, this column offers lower backpressure compared to smaller particle columns, allowing higher flow rates and broader system compatibility while maintaining dependable performance.

50 mm Length – Fast Screening and Rapid Methods

The 50 mm length is optimized for speed, making it ideal for rapid analysis and high‑throughput environments.

• Very fast run times for screening applications

• Reduced solvent consumption

• Efficient method development starting point

✅ Recommended for high‑throughput labs and rapid LC‑MS workflows

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Designed for low‑flow applications with improved pressure tolerance compared to smaller particles

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Lower backpressure than 3 µm columns

• Compatible with LC‑MS and low‑flow HPLC systems

• Improved robustness for routine use

✅ Important: Standard HPLC systems must be capable of stable, accurate low flow rates

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

60 Å Pore Structure

• Optimized for small molecule separations

• Increased interaction efficiency for lower molecular weight compounds

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak shape and symmetry

Optimized Efficiency (5 µm Particles)

• Lower backpressure vs 3 µm columns

• Reliable and robust performance

High Purity Silica

• Consistent chromatographic results

• Reproducible retention and selectivity

Typical Applications

• Halogenated compound screening

• Aromatic compound separations

• Rapid method development

• LC‑MS screening workflows

• USP L43 methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for LC‑MS and low‑flow HPLC systems (2.1 mm ID)

• Lower backpressure compared to smaller particle sizes

• Use 0.2 µm filtration prior to injection